mGeCKO Metabolic Library#1 in 4T1

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mGeCKO Metabolic Library#1 in 4T1

Mouse CRISPR Metabolic Gene Knockout Library

Product Name：mGeCKO Metabolic Library#1 in 4T1

Cell Line：4T1

Catalog#： LIBR-M001A-C300D248

Size(Applicable Cell Coverage) ： 300x

Instruction：

Order Now
Detailed Product Information Recommended Companion Products
Ubigene's CRISPR Library Cell Pool is conducted by utilizing CRISPR iScreen™. Ubigene's CRISPR Library Cell Pool is standardized constructed in batches by firstly obtaining Library Plasmid with high coverage and good uniformity using self-developed high-efficiency competent cells, then packaging the virus using Lentiviral Packaging Kit (#YK-LVP-20) to obtain high-titer CRISPR Library Virus, finally through the exclusive cell pool preparation process, infecting the target cell line with low infect MOI to restrict one virus per cell. Ubigene's CRISPR Library Cell Pools have small batch differences and good reproducibility. Screening on Cell Pools under different pressure conditions can screen target genes suitable for research in different fields.
Library Name
Mouse CRISPR Metabolic Gene Knockout Library
Product Name
mGeCKO Metabolic Library#1 in 4T1
Cell Line
4T1
Species
Mouse
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puromycin 1.5μg/mL
Culture Mediums
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Targeted Genes
2865
gRNA Number
22909
Non-targeting gRNA Number
50
Cell Image
Cell Reception
EZ-editor™ CRISPR Library Cell Pool will be transported in the form of cryopreserved cells. Upon received, immediately transfer to liquid nitrogen for storage or store briefly(24H) at-80°C freezer, or proceed directly to cell thawing.
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
FAQs
1
How to quality control library cell pools, and what are the quality control standards?
Quality control of library cell pools should involve NGS sequencing to assess sgRNA coverage, generally requiring coverage greater than 90%. Additionally, Ubigene’s library cell pools will undergo bacteria and mycoplasma detection, and post-thawing viability validation to ensure high-quality deliverables of library cell pools.
2
Why is the MOI for 30% virus infection efficiency chosen as the viral infection condition for library cell construction experiments?
During library cell construction, it is important to ensure that each cell receives only one sgRNA. A survival rate of 30% indicates that approximately 30% of the cells are infected by the virus, which greatly reduces the probability of multiple viruses infecting a single cell.
3
Can delivered library cell pools be further expanded?
Library cell pools can be further expanded, but it is recommended not to exceed 5 passages, as excessive passages may lead to a decrease in sgRNA coverage in the library cells.
4
During the expansion of cell pools or pressure screening, should antibiotics be added for maintenance?
During the expansion of library cell pools, antibiotic selection can be employed to ensure that no wild-type cells exist in the expanded cells. However, during the pressure screening, it is not recommended to use antibiotic selection to prevent antibiotics from affecting the experimental results.
5
How to calculate the number of wild-type cells needed for virus infection?
The calculation method for the amount of wild-type cells required for library virus infection is as follows: Required wild-type cell amount = gRNA number * gRNA coverage / Virus infection efficiency(30%)
6
How to ensure sgRNA coverage in poorly infectable library cells?
1.Optimize virus infection methods (e.g., centrifugation infection, adding transduction enhancers) to reduce virus infection MOI. 2.Conduct pre-experiments to explore virus infection efficiency and determine a suitable virus infection MOI.
7
Why is a pre-experiment necessary for drug screening?
The purpose of pre-experimentation in drug screening is to determine the appropriate drug screening concentration, avoiding too low concentrations that have no significant kill effect on cells or too high concentrations that lead to massive cell death, making it difficult to collect sufficient cells for subsequent NGS sequencing analysis.
8
What is the recommended duration for drug screening, and should positive or negative selection be chosen for drug-resistant cell screening?
A duration of no less than two weeks is generally recommended for drug screening. For knockout libraries, the enriched genes in the negative selection of NGS sequencing analysis are potential drug-resistance gene targets; for activation libraries, the enriched genes in the positive selection are potential drug-resistance gene targets.
9
What are the methods for screening library cells?
Methods for screening library cells include passaging culture, drug screening, virus infection, flow sorting, and in vivo screening, etc.

Related Popular Products

CRISPR-iScreen™ Library Plasmid

  • Mouse CRISPR Metabolic Gene Knockout Library
      inquiry   More details

    Library Type: Knockout Library
    Catalog#: LIBR-M003-P002;LIBR-M003-P100;LIBR-M003-P200;LIBR-M003-P500
    Size: 2ug;100ug;200ug;500ug
    Instruction: Mouse CRISPR Metabolic Gene Knockout Library

CRISPR-iScreen™ Library Virus

  • Mouse CRISPR Metabolic Gene Knockout Library
      inquiry   More details

    Library Type: Knockout Library
    Catalog#: LIBR-M003-LV1000;LIBR-M003-LV5000;LIBR-M003-LV10000
    Size: 1*10^8TU;5*10^8TU;1*10^9TU
    Instruction: Mouse CRISPR Metabolic Gene Knockout Library

CRISPR-iScreen™ Cell Pool

Related Service Recommendation

CRISPR Library Screen Service
Provides one-stop solutions for CRISPR-KO, CRISPRa, CRISPRi library screen from high-throughput sgRNA library construction, virus packaging, cell infection, drug screening, NGS sequencing to data analysis, etc. Various deliverables fulfill different research needs.
Learn More >
mGeCKO Metabolic Library#1 in 4T1

Mouse CRISPR Metabolic Gene Knockout Library

Product Name：mGeCKO Metabolic Library#1 in 4T1

Cell Line：4T1

Catalog#： LIBR-M001A-C300D248

Size(Applicable Cell Coverage) ： 300x

Instruction：

Order Now
Detailed Product Information Recommended Companion Products
Ubigene's CRISPR Library Cell Pool is conducted by utilizing CRISPR iScreen™. Ubigene's CRISPR Library Cell Pool is standardized constructed in batches by firstly obtaining Library Plasmid with high coverage and good uniformity using self-developed high-efficiency competent cells, then packaging the virus using Lentiviral Packaging Kit (#YK-LVP-20) to obtain high-titer CRISPR Library Virus, finally through the exclusive cell pool preparation process, infecting the target cell line with low infect MOI to restrict one virus per cell. Ubigene's CRISPR Library Cell Pools have small batch differences and good reproducibility. Screening on Cell Pools under different pressure conditions can screen target genes suitable for research in different fields.
Show More

Product Information

Library Name
Mouse CRISPR Metabolic Gene Knockout Library
Product Name
mGeCKO Metabolic Library#1 in 4T1
Cell line
4T1
Organism
Mouse
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puromycin 1.5μg/mL
Culture medium
88%RPMI-1640+10% FBS+1%Glutamax+1%Sodium Pyruvate
Targeted Genes
2865
gRNA Number
22909
Non-targeting gRNA Number
50
Cell Reception
EZ-editor™ CRISPR Library Cell Pool will be transported in the form of cryopreserved cells. Upon received, immediately transfer to liquid nitrogen for storage or store briefly(24H) at-80°C freezer, or proceed directly to cell thawing.
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
FAQs
1
How to quality control library cell pools, and what are the quality control standards?
Quality control of library cell pools should involve NGS sequencing to assess sgRNA coverage, generally requiring coverage greater than 90%. Additionally, Ubigene’s library cell pools will undergo bacteria and mycoplasma detection, and post-thawing viability validation to ensure high-quality deliverables of library cell pools.
2
Why is the MOI for 30% virus infection efficiency chosen as the viral infection condition for library cell construction experiments?
During library cell construction, it is important to ensure that each cell receives only one sgRNA. A survival rate of 30% indicates that approximately 30% of the cells are infected by the virus, which greatly reduces the probability of multiple viruses infecting a single cell.
3
Can delivered library cell pools be further expanded?
Library cell pools can be further expanded, but it is recommended not to exceed 5 passages, as excessive passages may lead to a decrease in sgRNA coverage in the library cells.
4
During the expansion of cell pools or pressure screening, should antibiotics be added for maintenance?
During the expansion of library cell pools, antibiotic selection can be employed to ensure that no wild-type cells exist in the expanded cells. However, during the pressure screening, it is not recommended to use antibiotic selection to prevent antibiotics from affecting the experimental results.
5
How to calculate the number of wild-type cells needed for virus infection?
The calculation method for the amount of wild-type cells required for library virus infection is as follows: Required wild-type cell amount = gRNA number * gRNA coverage / Virus infection efficiency(30%)
6
How to ensure sgRNA coverage in poorly infectable library cells?
1.Optimize virus infection methods (e.g., centrifugation infection, adding transduction enhancers) to reduce virus infection MOI. 2.Conduct pre-experiments to explore virus infection efficiency and determine a suitable virus infection MOI.
7
Why is a pre-experiment necessary for drug screening?
The purpose of pre-experimentation in drug screening is to determine the appropriate drug screening concentration, avoiding too low concentrations that have no significant kill effect on cells or too high concentrations that lead to massive cell death, making it difficult to collect sufficient cells for subsequent NGS sequencing analysis.
8
What is the recommended duration for drug screening, and should positive or negative selection be chosen for drug-resistant cell screening?
A duration of no less than two weeks is generally recommended for drug screening. For knockout libraries, the enriched genes in the negative selection of NGS sequencing analysis are potential drug-resistance gene targets; for activation libraries, the enriched genes in the positive selection are potential drug-resistance gene targets.
9
What are the methods for screening library cells?
Methods for screening library cells include passaging culture, drug screening, virus infection, flow sorting, and in vivo screening, etc.

Related Popular Products

CRISPR-iScreen™ Library Plasmid

  • Mouse CRISPR Metabolic Gene Knockout Library
      inquiry   More details

    Library Type: Knockout Library
    Catalog#: LIBR-M003-P002;LIBR-M003-P100;LIBR-M003-P200;LIBR-M003-P500
    Size: 2ug;100ug;200ug;500ug
    Instruction: Mouse CRISPR Metabolic Gene Knockout Library

CRISPR-iScreen™ Library Virus

  • Mouse CRISPR Metabolic Gene Knockout Library
      inquiry   More details

    Library Type: Knockout Library
    Catalog#: LIBR-M003-LV1000;LIBR-M003-LV5000;LIBR-M003-LV10000
    Size: 1*10^8TU;5*10^8TU;1*10^9TU
    Instruction: Mouse CRISPR Metabolic Gene Knockout Library

CRISPR-iScreen™ Cell Pool

Related Service Recommendation
CRISPR Library Screen Service
Provides one-stop solutions for CRISPR-KO, CRISPRa, CRISPRi library screen from high-throughput sgRNA library construction, virus packaging, cell infection, drug screening, NGS sequencing to data analysis, etc. Various deliverables fulfill different research needs.
Learn More >

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